《植物生理学报》 2013, 49(9): 959-967

不同耐淹油菜品种的耐淹性生理差异

吕艳艳, 金岩, 付三雄, 戚存扣*

江苏省农业科学院经济作物研究所, 南京210014

通信作者：戚存扣；E-mail: qckjaas@gmail.com；Tel: 025-84390372

摘　要：

以甘蓝型油菜耐淹品系WR-4和不耐淹品系WR-24为材料, 研究淹水处理后两个品系的植株形态差异、植株可溶性糖和脯氨酸的含量以及SOD活性和MDA含量的变化差异。结果表明, WR-4植株根系发育良好, 有支细根发生, 淹水6 d后, WR-4的根长增加了9.4%, WR-24的根长没有明显增加; 淹水6 d+去水后3 d, WR-24的干重增长幅度明显低于WR-4; 淹水6 d+去水后1 d, WR-4叶片中可溶性糖的含量和脯氨酸的含量增加, 且高于WR-24; WR-4叶片中氧化产物MDA的含量去水后逐渐下降, 而WR-24中MDA含量仍在增加, 相当于对照的2倍。通过组织化学染色, WR-4叶片中积累的过氧化氢(H2O2)和超氧阴离子(O2·ˉ)较WR-24少, 抗氧化酶SOD的活性明显高于WR-24。上述结果表明: 淹水胁迫下, WR-4通过增加可溶性糖及脯氨酸等渗透调节有机物的含量和及时启动抗氧化酶系统来恢复淹水伤害的能力显著高于不耐淹品系WR-24。

关键词：甘蓝型油菜; 耐淹性; 生理差异; SOD; MDA

收稿：2013-07-04   修定：2013-08-06

资助：国家油菜产业技术体系(CARS-13)和科技部支撑计划(2010-BAD01B10)。

Physiological Differences of Different Waterlogging-Resistant Brassica napus L. under Waterlogging Stress

LÜ Yan-Yan, JIN Yan, FU San-Xiong, QI Cun-Kou*

Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China

Corresponding author: QI Cun-Kou; E-mail: qckjaas@gmail.com; Tel: 025-84390372

Abstract:

The morphological performance and physiological mechanism of Brassica napus under water stress were studied by waterlogging (submergence) treatment of the seedlings of waterlogging-resistant line WR-4 and waterlogging-susceptible line WR-24. The contents of soluble sugar and proline, and activities of SOD and MDA content in the seedlings were measured. Results showed that after submergence treatment, WR-4 performed a well-developed root system with more branch hair on the main root. The root length of WR-4 increased by 9.4% compared to control. The dry weight of WR-4 from 6 d submergence+3 d recovery treatment increased more than WR-24. The soluble sugar and proline content in leaf of WR-4 from 6 d submergence+1 d recovery treatment increased more than WR-24. Content of MDA in leaves of WR-4 decreased after 6 d submergence+1 d recovery, while that in WR-24 was increasing. Through histochemical staining, WR-4 accumulated less hydrogen peroxide (H2O2) and superoxide anion (O2¯·) in leaves comparing to WR-24. And its SOD activity was significantly higher than WR-24. It indicated that WR-4, the waterlogging-resistant line showed a strong ability to restore damage resulting from water stress by increasing the content of soluble sugar and proline, and timely starting antioxidant enzyme system to defend against oxidative damage.

Key words: Brassica napus L.; waterlogging-resistant; physiological differences; SOD; MDA